Molecular detection of Tick-Borne Pathogens in Ovaries and a Mummified Foetus of three cows with Historical Reproductive Problems in Northern Veracruz, Mexico

Abstract

The aim of this study was to identify the presence of tick-borne pathogens as the probable cause of reproductive problems in cows in a commercial dual-purpose cow operation living in tropical conditions in Northern Veracruz, Mexico. As part of the reproductive control of the herd, ultrasonography was performed periodically, and the presence of three open (non-pregnant) cows with historical infertility problems was detected. The ovaries from the two open cows and the mummified foetus of a third one were removed, and two sets of tissue samples were collected. Histopathology analysis revealed the presence of follicular abnormalities. The DNA tests revealed the presence of Anaplasma marginale, Babesia bigemina and/or Babesia bovis. In conclusion, the present work represents the first report of tick-borne pathogens in ovarian tissue samples and a mummified foetus of infertile cows in tropical conditions in Northern Veracruz, Mexico, highlighting the possibility that many reproductive problems in tropical conditions could be caused by tick-borne pathogens.